Identification and Characterization of Azo Dye Decolourizing Bacterial Strains, Alcaligenes faecalis E5.Cd and A. faecalis Fal.3 Isolated from Textile Effluents
Keywords:
Textile effluent, Azo dye, Decolourization, Alcaligenes faecalis.Abstract
The study was designed for isolation and characterization of azo dye decolourizing bacteria which is a prerequisite for developing a microorganism-facilitated treatment of polluting dyes. In this study nine types of bacteria which were able to decolourize three types of azo dyes (Blue H/C, Red 3B and Yellow 3R dye) were isolated from textile effluents collected from Gazipur industrial area in Bangladesh. Depending on 16S rDNA analysis, the most efficient decolourizing bacterium for the Blue H/C and the Red 3B dye was identified as Alcaligenesfaecalis strain E5.Cd while that for the Yellow 3R dye was identified as Alcaligenesfaecalis strain Fal.3. After characterization, both A. faecalis E5.Cdand A. faecalis Fal.3 were found to grow optimally at 35 0C and at pH 7 and pH 8, respectively. Both of these strains were sensitive to all antibiotics studied except for Bacitracin. Also, both strains showed maximum decolourization activities after 96 hours incubation in MS media at pH 7 (up to 93%) and pH 8 (up to 94%), at 35 0C temperature ( up to 91%), at 50 ppm initial dye concentration (up to 92%), at 20% inoculum size (up to 93%), and at supplementation of 1% co-substrate (up to 93%).
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[2] M. S. Sultana, M. S. Islam, R. Saha, and M. Al-Mansur, "Impact of the effluents of textile dyeing industries on the surface water quality inside DND embankment, Narayanganj," Bangladesh Journal of Scientific and Industrial Research, vol. 44, pp. 65-80, 2009.
[3] E. Tom-Sinoy, D. A. Mohan, and H. Shaikh, "Biodegradation of textile dyes by Pseudomonas species and E. coli," VSRD Technical and Non-Technical Journal 2 (5): 238, vol. 248, 2011.
[4] P. C. Vandevivere, R. Bianchi, and W. Verstraete, "Review: Treatment and reuse of wastewater from the textile wet?processing industry: Review of emerging technologies," Journal of Chemical Technology and Biotechnology, vol. 72, pp. 289-302, 1998.
[5] M. Vijaykumar, P. A. Vaishampayan, Y. S. Shouche, and T. Karegoudar, "Decolourization of naphthalene-containing sulfonated azo dyes by Kerstersia sp. strain VKY1," Enzyme and Microbial Technology, vol. 40, pp. 204-211, 2007.
[6] T.-H. Kim, Y. Lee, J. Yang, B. Lee, C. Park, and S. Kim, "Decolorization of dye solutions by a membrane bioreactor (MBR) using white-rot fungi," Desalination, vol. 168, pp. 287-293, 2004.
[7] A. Pandey, P. Singh, and L. Iyengar, "Bacterial decolorization and degradation of azo dyes," International Biodeterioration & Biodegradation, vol. 59, pp. 73-84, 2007.
[8] N. Daneshvar, M. Ayazloo, A. Khataee, and M. Pourhassan, "Biological decolorization of dye solution containing Malachite Green by microalgae Cosmarium sp," Bioresource technology, vol. 98, pp. 1176-1182, 2007.
[9] N. Dafale, L. Agrawal, A. Kapley, S. Meshram, H. Purohit, and S. Wate, "Selection of indicator bacteria based on screening of 16S rDNA metagenomic library from a two-stage anoxic–oxic bioreactor system degrading azo dyes," Bioresource technology, vol. 101, pp. 476-484, 2010.
[10] H. Saunders, T. O'Brien, and R. Nixon, "Textile dye allergic contact dermatitis following paraphenylenediamine sensitization from a temporary tattoo," Australasian journal of dermatology, vol. 45, pp. 229-231, 2004.
[11] K. Sasaki, M. Sakai, K. Matusita, Y. Masuda, and K. Sato, "Chemical structure analysis for azo type disperse dyes by mass spectroscopy and detection of dyestuff in textile products causing allergic contact dermatitis," Bunseki Kagaku, vol. 57, pp. 833-850, 2008.
[12] J. Knapp, P. Newby, and L. Reece, "Decolorization of dyes by wood-rotting basidiomycete fungi," Enzyme and Microbial Technology, vol. 17, pp. 664-668, 1995.
[13] C. J. Ogugbue, N. Morad, T. Sawidis, and N. A. Oranusi, "Decolorization and partial mineralization of a polyazo dye by Bacillus firmus immobilized within tubular polymeric gel," 3 Biotech, vol. 2, pp. 67-78, 2012.
[14] M. K. Hafshejani, C. J. Ogugbue, and N. Morad, "Application of response surface methodology for optimization of decolorization and mineralization of triazo dye Direct Blue 71 by Pseudomonas aeruginosa," 3 Biotech, vol. 4, pp. 605-619, 2014.
[15] F. Anwar, S. Hussain, S. Ramzan, F. Hafeez, M. Arshad, M. Imran, et al., "Characterization of reactive red-120 decolorizing bacterial strain Acinetobacter junii FA10 capable of simultaneous removal of azo dyes and hexavalent chromium," Water, Air, & Soil Pollution, vol. 225, pp. 1-16, 2014.
[16] S. V. Surwase, K. K. Deshpande, S. S. Phugare, and J. P. Jadhav, "Biotransformation studies of textile dye Remazol Orange 3R," 3 Biotech, vol. 3, pp. 267-275, 2013.
[17] L. Kumari, A. K. Verma, D. Tiwary, D. D. Giri, G. Nath, and P. K. Mishra, "Biodegradation of Navy N5RL1 carpet dye by Staphylococcus saprophyticus strain BHUSS X3," 3 Biotech, vol. 5, pp. 775-782, 2015.
[18] A. R. Santal, N. Singh, and B. S. Saharan, "Biodegradation and detoxification of melanoidin from distillery effluent using an aerobic bacterial strain SAG 5 of Alcaligenes faecalis," Journal of hazardous materials, vol. 193, pp. 319-324, 2011.
[19] P. D. Shah, S. R. Dave, and M. Rao, "Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain Alcaligenes faecalis PMS-1," International Biodeterioration & Biodegradation, vol. 69, pp. 41-50, 2012.
[20] J. Wiegel, X. Zhang, and Q. Wu, "Anaerobic dehalogenation of hydroxylated polychlorinated biphenyls by Desulfitobacterium dehalogenans," Applied and environmental microbiology, vol. 65, pp. 2217-2221, 1999.
[21] R. Saratale, G. Saratale, D. Kalyani, J.-S. Chang, and S. Govindwar, "Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR," Bioresource technology, vol. 100, pp. 2493-2500, 2009.
[22] G. Smith, S. Socransky, and C. Smith, "Rapid method for the purification of DNA from subgingival microorganisms," Oral microbiology and immunology, vol. 4, pp. 47-51, 1989.
[23] A. Saha, M. Haque, S. Karmaker, and M. Mohanta, "Antibacterial effects of some antiseptics and disinfectants," Journal of Life and Earth Science, vol. 3, pp. 19-21, 2009.
[24] S. Mutafov, T. Avramova, L. Stefanova, and B. Angelova, "Decolorization of acid orange 7 by bacteria of different tinctorial type: a comparative study," World Journal of Microbiology and Biotechnology, vol. 23, pp. 417-422, 2007.
[25] K. Kumar, S. S. Devi, K. Krishnamurthi, D. Dutta, and T. Chakrabarti, "Decolorisation and detoxification of Direct Blue-15 by a bacterial consortium," Bioresource technology, vol. 98, pp. 3168-3171, 2007.
[26] C. O'neill, A. Lopez, S. Esteves, F. Hawkes, D. Hawkes, and S. Wilcox, "Azo-dye degradation in an anaerobic-aerobic treatment system operating on simulated textile effluent," Applied microbiology and biotechnology, vol. 53, pp. 249-254, 2000.
[27] S. D. Ashrafi, S. Rezaei, H. Forootanfar, A. H. Mahvi, and M. A. Faramarzi, "The enzymatic decolorization and detoxification of synthetic dyes by the laccase from a soil-isolated ascomycete, Paraconiothyrium variabile," International Biodeterioration & Biodegradation, vol. 85, pp. 173-181, 2013.
[28] R. Khan, P. Bhawana, and M. Fulekar, "Microbial decolorization and degradation of synthetic dyes: a review," Reviews in Environmental Science and Bio/Technology, vol. 12, pp. 75-97, 2013.
[29] J. Guo, J. Zhou, D. Wang, C. Tian, P. Wang, and M. S. Uddin, "A novel moderately halophilic bacterium for decolorizing azo dye under high salt condition," Biodegradation, vol. 19, pp. 15-9, Feb 2008.
[30] M. Kesik, S. Blagodatsky, H. Papen, and K. Butterbach?Bahl, "Effect of pH, temperature and substrate on N2O, NO and CO2 production by Alcaligenes faecalis p," Journal of Applied Microbiology, vol. 101, pp. 655-667, 2006.
[31] K.-C. Chen, J.-Y. Wu, D.-J. Liou, and S.-C. J. Hwang, "Decolorization of the textile dyes by newly isolated bacterial strains," Journal of Biotechnology, vol. 101, pp. 57-68, 2003.
[32] S. Asad, M. Amoozegar, A. A. Pourbabaee, M. Sarbolouki, and S. Dastgheib, "Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria," Bioresource technology, vol. 98, pp. 2082-2088, 2007.
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2017-05-13
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Saha, A. K., Sultana, N., Mohanta, M. K., Mandal, A., & Haque, M. F. (2017). Identification and Characterization of Azo Dye Decolourizing Bacterial Strains, Alcaligenes faecalis E5.Cd and A. faecalis Fal.3 Isolated from Textile Effluents. American Scientific Research Journal for Engineering, Technology, and Sciences, 31(1), 163–175. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/2889
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