Antifungal Activity of Lactic Acid Bacteria Isolated from Soil Rhizosphere on Fusarium Species Infected Chilli Seeds
Lactic acid bacteria (LAB) are known to inhibit the growth of fungi and LAB cells or its can be used as biocontrol against plant pathogens. The aim of this study was to evaluate the efficacy of selected LAB strains isolated from rhizospheric soil against Fusarium spp. These fungi were isolated from infected different plant parts and identified as F. oxysporum strain KAML01, Fusarium sp. CID124, Fusarium sp. fus 124 and F. proliferatum with 99 % similarity using Internal Transcribed Spacer (ITS) analysis. Five LAB strains were showed strong antifungal activity against the Fusarium spp. were evaluated using dual overlay method. Isolates MSS1 and FCF11 were 100% similarity with Lactobacillus plantarum and isolate MSS5 99% was similarity with Pediococcus pentoceous using 16S rDNA sequence analysis. Inhibition of Fusarium sp. CID124 fungal biomass in the de Man, Rogosa and Sharpe Broth (MRSB) was significantly greater (P ≤ 0.05) by cells of P. pentosaceus (97.43%) compared to inhibition of Fusarium sp. CID124 fungal biomass by Cell Free Supernatant (CFS) of Lb.
acidophilus ATCC314 (91.8%) using biomass dry method in Malt Extract Broth (MEB) medium. Fusarium spp. infected to seeds of three cultivars, Cili Kulai, Red spicy-0639 and GAAC-SEED-5005 and then, treated with CFS-LAB. All treated seeds with CFS-LAB showed good germination in the range of 82.6 to 93.3% compared to seed germination 53.3 to 70.0% of chili seeds infected with Fusarium spp. The percentage germination of chilli seeds was strongly suppressed when infected with Fusarium sp. CID124. This infection had significant (P≤0.05) reduction of seed germination to 53.3 % compared to other Fusarium spp. Finally, the pathogenicity effect of Fusarium sp. CID124-CS was highly virulent on seeds germination of chilli.
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