Study on Sludge Characteristics and Nitrogen Removal Mechanism of Hybrid Partial Nitrification Anammox Reactor (HPNA)

Authors

  • MD. Asadur Rahoman Northeastern University, NO. 3-11, Wenhua Road, Heping District, Shenyang, 110819, P. R. China
  • Chai Tianyu Northeastern University, NO. 3-11, Wenhua Road, Heping District, Shenyang, 110819, P. R. China
  • Zhu Tong Northeastern University, NO. 3-11, Wenhua Road, Heping District, Shenyang, 110819, P. R. China

Keywords:

Partial nitrification, anammox, hybrid reactor, sludge characteristics, nitrogen removal mechanism.

Abstract

Anaerobic ammonium oxidation (anammox) is a chemical process that involves the removal of inorganic nitrogen during the treatment of wastewater. Partial nitrogen-anammox process is the best alternative to the conventional nitrification-denitrification process in terms of cost-effectiveness. During the process, half of the ammonium is partly oxidized into nitrite. An Anammox bacterium is oxidized by ammonium to nitrogen gas with the use of an electron acceptor (nitrite) under anoxic conditions, and their growth occurs by carbon dioxide fixation. The process of partial nitritation-anammox demands no exogenous carbon source (e.g. methanol) and less oxygen as compared to the conventional nitrification-denitrification process. Another factor that lowers the operational costs is the low sludge production. Nevertheless, since there is low biomass production of anammox bacteria, the reactors that effectively retain biomass and can provide a long solids retention time are needed for the anammox process to be efficient and successful. A single reactor usage has a number of benefits to the process setup. Single–stage processes have easy reactor configuration and lower operational costs.  In this study, the hybrid anammox reactor has two types of reactors developed to be used, the fluidized and fixed beds.

The following had to undergo experimental evaluation which has vital theoretical and practical significance

  • The rapid startup of the anammox systems
  • Enrichment of anammox granular
  • The best nitrogen removal performances

References

[1]. Mulder, A.,van de Graaf, A.A., Robertson, L.A; Kuenen, J.G., 1995, Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor, FEMS.Microbiol. Ecol., 16, 177-184.
[2]. Van de Graaf, A.A, De Bruijin, L.A.P., Jetten, M.S.M., Kuenen,J.G., Anaerobic oxidation of ammonium is a biologically mediated process, Appl. Microbiol., 61, 1246-1251.
[3]. Van de Graaf, A.A, De Bruijin, P., Robertson, L.A., Jetten , M.S.M., Kuenen, J.G.,
1996, Autotrophic growth of anaerobic ammonium-oxidizing microorganinms in a fluidizing bed reactor,Appl.En.zviron.Microbiol., 142,2187-2196.
[4]. Sliekers, A.O., Third,k; Abma,W., Kuenen, J.G., Jetten, M.S.M., 2003,canon and anammox in a gas-lifted reactor, FEMS. Microbiol.Lett., 218,339-344.
[5]. Strous, M., van Gerven, E., Kuenen, J.G., Jetten, M., 1997, Ammonium removal from concentrated waste streams with the anaerobic ammonium oxidation (Anammox ) process in different reactor configurations, Water Res.,31,1955-1962.
[6]. Strous,M., Kuenen, J.G., Jetten, M.S.M., 1999, Key physiological parameters of anaerobic ammonium oxidation , Appl. Environ. Microbiol., 65, 3248-3250.
[7]. Dapena-Mora A., Arroja B., Campus J.L., Mosquera-Corral A. and Mendez R., 2004, Improvement of the settling properties of anammox sludge in an SBR,j. Chen.Technol. Biotechnol.,79,1417-1420.
[8]. Furukawa,K., Rouse, J.D., Imajo, U., Nakamura, K, Ishida, H., 2001, Anaerboic oxidation of ammonium confirmed in continuous flow treatment using a non-woven biomass carrier,Jpn.J.of water Treatment Biology,38(2), 87-94.
[9]. Zhang, L., Yang, J., Ma,Y., Li, Z., Fujii,T., Zhang, W., Nishiyama, T., and Furukawa, K., 2010, Treatment capacity of an up-flow anammox column reactor using polyethylene sponge strips as biomass carrier, J. Biosci. Bioeng., 110 (1), 72-78.
[10]. Isaka, K., Date, Y., Sumino,T., Tsuneda, S., 2007, Ammonium removal performance of anaerobic ammonium-oxidizing bacteria immobilized in polyethylene glycol gel carrier: anammox bacteria immobilized in gel carrier, App. Environ. Microbiol., 76 (6), 1457-65.
[11]. Hoa, T.T.H., Khanh, L.N., Liu, Z., Fujii,T., Rouse,J.D., Furukawa, K., 2006, Nitrogen removal by immobilized anammox sludge using PVA gel as biocarrer, Jpn. J. of Water Treatment Biology, 42 (3), 139-149.
[12]. Qiao, S., Kawakubo,Y., Chen, Y., Nishiyama, T., Fujii, T., Furukawa, k., 2009, Identification of bacteria coexisting with anammox bacteria in an uplow column type reactor, Biodegradation,20 ,117-124
[13]. Colleran, E., Finnegan, S., Okeeffe,R.B., 1994, Anaerobic digestion of high sulphate containing wastewater from the industrial production of citric acid, Wat. Sci. Technol.,30,263-73.
[14]. Ozturk, I., Eroglu, V., Ubay,G., Demir,I., 1993, Hybrid upflow anaerobic sludge blanket reactor (HUASBR) treatment of dairy emuents, Wat. Sci.Technol., 28, 77-85.
[15]. Boja, R., Banks,C.J., Wang, Z., 1995, performance of a hybrid anaerobic reactor, combining a sludge blanket and a filter, treating slaughterhouse wastewater, Appl. Microbiol. Biotechnol., 43,351-357
[16]. Fujii,T., Sugino,H., Rouse, J .D., Furukawa,K., 2002, Characterization of the microbial community in an anaerobic ammonium-oxidizing bioflim cultured on a nonwoven biomass carrier, J. Biosci.Bioeng., 94, 412-418.
[17]. Furukawa, k., Rouse, J.D., yoshida, N., H., 2003, Mass cultivation of anaerobic ammonium-oxidizing sludge using a novel nonwoven biomass carrier, J. Chen. Eng. Of Jpm., 36 (10),1163-1169.
[18]. Kanda, J., 1995,Determination of ammonium in seawater based on the indophenol reaction with o- phenylphenol(oop),Water res., 29, 2746-2750.
[19]. APHA,AWWA,WPCF: Standard methods for the examination of water and wastewater,19thedition,American public Health Association,Washington,D.C.(1995)
[20]. Yang, J., Zhang,L;Fukuzaki,Y., Hira,D., Fukuzaki,Y., Furukawa, K., inorganiccarbonsource2010,High-rate nitrogen removal by the anammox process with a sufficient,Bioresource.Technol., 101,9471-1251.
[21]. Liu,S., Yang, F., Xue, Y., Gong, Z., Chen, H., Wang, T., Su, Z., 2008, Evaluation of oxygen adaptation and identification of functional bacteria composition for Anammox consortium in non-woven biological rotating contactor,Bioresour. Technol., 99,8273-8279.
[22]. Tsushima, I., Ogasawara,Y., Kindaichi, T., Okabe, S., 2007,Development of high-rate anaerobic ammonium-oxidizing (Anammox) biofilm reactors, Water Res., 41, 1623-1634.
[23]. Tang,C.J;Zheng,p; Hu,B.L; Chen,J.G; Wang.C.H;2010, Influence of substrates on nitrogen removal performance and microbiology of anaerobic ammonium oxidation by operation two USAB reactors fed with different substrate levels,J.Hazard. Mater;181,19-26.
[24]. Tang, C.J., Zheng, p., Wang, C.H., Mahmoo, Q., Zhang, J.Q., Chen, X.G., Zhang,L., Chen, J.W., 2011, Performance of high –loaded ANAMMOX UASB reactors containing granular sludge, Water Res., 45,135-144.
[25]. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., 1951, Protein measurement with the foil phenol reagent Journal of Biological Chemistry, 193, 265-275.
[26]. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F., 1956, Colorimetric method for determination of sugars and related substances, Analytical Chemistry, 28 (3), 350-356.
[27]. Anthonism A.C., Loehr R.C., Prakasam T.B.S., Srinath E.G., 1976, Inhibition of nitrification by ammonia and nitrous acid, J. Water pollut. Control Fed., 48 (5), 835-852.
[28]. Sharma B., Ahler R.C., 1977, Nitrification and nitrogen removal, Water Research, 11, 897-925.
[29]. Vadivelu V.M., Keller J., Yuan Z.G., 2007, Effect of free ammonia on the respiration and growth processes of an enriched Nitrobacter culture, Water Research, 41 (1), 826-834.
[30]. Stuven R., Bock E., 2001, Nitrification and denitrification as a source for NO and NO2 production in high-strength wastewater, Water Research, 35(8), 1905-1914.
[31]. Quarmby, J., forster, C.F., 1995, An examination of the structure of UASB granules, Water Res., 29, 2449-2454.
[32]. Batstone, D.J., Keller, J., 2001, Variation of bulk properties of anaerobic granules with wastewater type, Water Res., 35.1723-1729.
[33]. Franco, A., Roca, E., Lema, J.M., 2006. Granulation in high-load denitrifying upflow sludge bed pulsed reactors, Water Res. 40, 871-880.
[34]. Wu, J., Zhou, H.M., Li, H.Z., Zhang, P.C., Jiang, J., 2009, Impacts of hydeodynamic shear force on nucleation of flocculent sludge in anaerobic reactor, Water Res., 3029-3036.
[35]. S.E. Vlaeminck,A. Terada, B.F. Smets, H.D.Clippeleir, T. Schaubroeck,S.Bolca, L. Demeestere,J. Mast, N. Boon, M. Carballa,W. Verstraete, Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox ,Appl.Environ.Microbiol.76 (2010)900-909.
[36]. E.M. Gilbert, E. Muller, H. Horn, S. Lackner, Microbial activity of suspended biomass from a nitritation-anammox in SBR in dependence of operational condition and size fraction,Appl. Microbial.Biotechnol.97 (2013) 8795-8804.
[37]. B. Wett, M.Hell, G. Nyhuis, T. Puempel, I. Takacs, S. Murthy, Syntrophy of aerobic and anaerobic ammonia oxidizers, Water Sci. Technol. 61 (2010) 1915-1922.
[38]. A. Joss, D. Salzgeber, J. Eugster, R .Kong, K.Rottermann, S. Leumann, J. Mohn, H. Siegrist, Full-scale nitrogen removal from digester liquid with partial nitritation and anammox in one SBR, Environ. Sci. Technol. 43 (2009) 5301-5306.
[39]. H. Park ,A, Rosenthal, R. Jezek, K. Ramalingam,J. Fillos, K. Chandran, Impact of inocula and growth mode on the molecular microbiology ecology of anaerobic ammonia oxidation (anammox) bioreactor communities, Water Res. 44 (2010) 5005-5306.
[40]. J.Vazquez-padin, A. Mosquera-Corral, J.L. Campos, R. Mendez, N.P. Revsbech, Microbial community distribution and activity dynamics of granular biomass in a CANON reactor, water Res.44 (2010) 4359-4370.
[41]. M. Oshiki, M. Shimokawa, N. Fujii, H. Satoh, S.Okabe, Physiological characteristics of the anaerobic ammonium-oxidizing bacterium ‘Candidatus Brocadia sinica’, Microbiology 157(2011) 1706-1713.
[42]. M.K . Winkler, R. Kleerebezem, M.C.M. van Loosdrecht, Integration of anammox into the aerobic granular sludge process for main stream wastewater treatment at ambient temperatures, Water Res. 46 (2012) 136-144.
[43]. H. Park, A. Rosenthal, K. Ramalingam, J. Fillos, K. Chandran, Linking community profiles, gene expression and N-removal in anammox bioreactors treating municipal anaerobic digestion reject water, Environ. Sci. Technol. 44 (2010) 6110-6116.
[44]. J. Li, B.Hu, P. Zheng, M. Qaisar, L. Mei, Filamentous granular sludge bulking in a laboratory scale UASB reactor, Bioresour. Technol. 99 (2008) 3431-3438.
[45]. L. Bjornsson, P. Hugenholtz, G.W. Tyson, LL blackall, Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal, Microbiology 148 (2002) 2309-2318.
[46]. C. Kragelund, C. Levantesi, A. Borger, K. Thelen, D. Eikelboom, V.Tandoi, Y. Kong, J. van der Waarde, J. Krooneman, S. Rssetti, T.R.Thomsen, P. H. Nielsen, Identity, abundance and ecophysiology of filamentus Chloroflexi species present in activated sludge treatment plants, FEMS Microbiol. Ecol. 59 (2007) 671-682.
[47]. D. Gao, L. Liu, H. Liang, W. Wu, Aerobic granular sludge: characterization, mechanism of granulation and application to wastewater treatment, C rit. Rev. Biotechnol. 31 (2011) 137- 152.
[48]. Y.Xia, Y. Kong, P.H, Nielsen, In situ detection of starch-hydrolyzing microorganism in activated sludge, FEMS Microbiol. Eco. 60 (2008) 156-165.
[49]. C. Kragelud, T.R. Thomsen, A.T. Mielczarek, P.H. Nielsen, Eikelboom’s morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum chloroflexi, FEMS Microbiol. Ecol. 76 (2006) 451-462.
[50]. T. Kindaichi, S.Yuri, N. Ozaki, A. Ohashi, Ecophysiological role and function of uncultured chloroflexi in an anammox reactor, Water Sci. Technol. 66(2012) 2556-2561

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Published

2017-03-19

How to Cite

Rahoman, M. A., Tianyu, C., & Tong, Z. (2017). Study on Sludge Characteristics and Nitrogen Removal Mechanism of Hybrid Partial Nitrification Anammox Reactor (HPNA). American Scientific Research Journal for Engineering, Technology, and Sciences, 29(1), 80–115. Retrieved from https://asrjetsjournal.org/index.php/American_Scientific_Journal/article/view/2638

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